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Masters Thesis, December 2000, 47pp. "The Search for Exudates from Eurasian Watermilfoil and Hydrilla" L.M. Glomski Plants are well known for their ability to produce secondary metabolites, compounds that benefit the plant by inhibiting growth of potential disease organisms, herbivore or competitors. Some secondary metabolites also have been shown to be toxic and/or carcinogenic to animals. Secondary metabolites are also produced by aquatic plants, and in some instances, exudation of these metabolites into the surrounding water has been detected. To determine whether infestations of Eurasian watermilfoil or hydrilla produce such exudates, plant tissues and water samples were collected from laboratory cultures and pond populations. These samples were analyzed using solid phase extraction, HPLC, and various methods of mass spectrometry including electrospray ionization, GC/MS, electron impact and chemical ionization. Previously reported compounds such as tellimagrandin II (Eurasian watermilfoil) and a caffeic acid ester (hydrilla), along with a newly discovered flavonoid, cyanidin 3-dimalonyl glucoside (hydrilla), were readily detected in plant tissues but were not detected in any of the water samples. If compounds are being released, as suggested by researchers using axenic cultures, they may be rapidly degraded by bacteria For more information: Glomski, Lee Ann
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